- Original Article
- Effect of growth hormone on neuronal death in hippocampal slice cultures of
neonatal rats exposed to oxygen-glucose deprivation
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Kyung Sik Hong, Jihui Kang, Myeung Ju Kim, Jeesuk Yu, Young Pyo Chang
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Clin Exp Pediatr. 2009;52(5):588-593. Published online May 15, 2009
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Purpose : To investigate whether growth hormone (GH) has a protective effect on neurons in hippocampal slice cultures of neonatal rats exposed to oxygen-glucose deprivation (OGD).
Methods : Cultured hippocampal slices of 7-day-old rats were exposed to OGD for 60 min. Then, the slices were immediately treated with three doses of GH (5, 50, or 500 µM) in media. The... |
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- Injury of neurons by oxygen-glucose deprivation in organotypic hippocampal slice culture
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David Chanwook Chung, Kyung Sik Hong, Jihui Kang, Young Pyo Chang
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Clin Exp Pediatr. 2008;51(10):1112-1117. Published online October 15, 2008
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Purpose : We intended to observe cell death and apoptotic changes in neurons in organotypic hippocampal slice cultures following oxygen-glucose deprivation (OGD), using propidium iodide (PI) uptake, Fluoro-Jade (FJ) staining, TUNEL staining and immunofluorescent staining for caspase-3.
Methods : The hippocampus of 7-day-old rats was cut into 350 µm slices. The slices were cultured for 10 d (date in vitro, DIV... |
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